Sourcing Guide · GLP-3 RT · Triple Incretin Agonist · Research Use Only

Where to Buy GLP-3 RT
for Research in 2026

Evo Peptides Research Team ~12 min read LY3437943 Research Use Only
99.91%
Max HPLC Purity (lot-specific)
3rd Party
Independent COA + MS
Same Day
Ships before 3 PM CST
Wisconsin
US Domestic Fulfillment
Bottom Line Up Front

GLP-3 RT (LY3437943) is available from US research peptide suppliers under Research Use Only (RUO) labeling for in vitro laboratory work. It is not FDA-approved for any therapeutic use, is not a controlled substance, and is not subject to the compounding pharmacy framework that governs prescription peptides. The single most important variable when sourcing it is COA quality — specifically whether the supplier provides independent third-party mass spectrometry to confirm molecular identity, not merely HPLC purity. Because no pharmacopeial reference standard exists for this compound, identity verification is more critical than for approved dual agonists such as GLP-2 TRZ.

Evo Peptides supplies GLP-3 RT in 15 mg, 20 mg, and 30 mg formats. Current lot-specific third-party HPLC purity values are 99.27%, 99.85%, and 99.91% respectively. Batch-specific third-party COAs (including mass spectrometry) are available for review before purchase. Fulfillment is from Wisconsin with same-day shipping on orders placed before 3:00 PM CST.

Research Use Only (RUO). This material is intended solely for in vitro laboratory research. It is not for human or veterinary administration, consumption, or any clinical use. These statements have not been evaluated by the FDA and are not intended to diagnose, treat, cure, or prevent any disease. Purchasers must be 21 years of age or older and acknowledge research-only intent.

What "Research Use Only" means legally

RUO labeling designates the product for in vitro laboratory research only. It is a legal use designation, not a loophole. Vendors market and sell under this classification; buyers acknowledge research-only intent at the time of purchase. The RUO channel is governed differently from the prescription compounding channel and does not authorize human or veterinary use.

The Core Challenge

Why sourcing GLP-3 RT requires higher scrutiny than most research peptides

Most long-established research peptides have undergone market selection: vendors producing poor-quality material tend to lose researcher trust over time. GLP-3 RT presents three compounding challenges that raise the analytical bar.

First, it is a relatively new synthesis target for many research suppliers. Interest following Phase 2 data brought in vendors with limited prior experience synthesizing lipidated 39-residue triple agonists. Synthesis errors can produce material that shows high HPLC purity yet contains incorrect sequence or missing lipid modification — failures that pass a purity screen but fail functional assays.

Second, no pharmacopeial reference standard exists for GLP-3 RT. Approved dual agonists benefit from FDA-recognized reference standards that independent laboratories can anchor identity confirmation to. For GLP-3 RT no such standard is available. Mass spectrometry of the intact sequence (including the C18 lipid) is therefore the definitive identity test. A COA that reports only HPLC purity leaves open the possibility of a pure but incorrect compound.

Third, the compound sits at the higher end of typical research-catalog pricing, which can create pressure to reduce analytical costs while keeping list prices competitive.

39Amino acids in the GLP-3 RT sequence — each position must be consistent with the expected mass
0FDA pharmacopeial reference standards for GLP-3 RT (unlike approved dual agonists)
≥99%Recommended minimum HPLC purity for research-grade material; the ≥95% floor is inadequate
COA Standards

What a legitimate GLP-3 RT Certificate of Analysis must contain

A Certificate of Analysis is the primary document that describes what is in a given vial. For many research peptides HPLC purity is a useful first filter. For GLP-3 RT it is insufficient by itself: without a reference standard, HPLC shows only that a pure substance is present, not that the substance is GLP-3 RT. For general guidance on interpreting peptide COAs, see how to read a peptide COA. Below are the minimum elements a sufficient GLP-3 RT COA should include, and common shortfalls that do not meet research-grade expectations.

✓ Required — all five should be present
HPLC purity ≥99% with chromatogram available Reported purity should be 99% or higher. Material at or below 95% can contain meaningful levels of truncation sequences and de-lipidated species that introduce functional noise. The chromatogram itself should be available so the reported value can be verified.
Mass spectrometry identity confirmation (ESI-MS or MALDI-TOF) Non-negotiable for GLP-3 RT. Confirmation that the observed molecular weight matches the expected mass for the intact sequence including the lipid modification (approximately 4,900 Da) is the practical way to confirm identity in the absence of a reference standard.
Independent third-party testing laboratory named on the certificate The testing facility should be a separate legal entity from the vendor, with publicly verifiable contact and accreditation information. Internal vendor QC certificates do not constitute independent verification.
Batch or lot number matching the vial label The COA must correspond to the specific production batch of the material received. A generic or mismatched lot number cannot be linked to the vial in hand.
Issue date within approximately 12 months Analytical results from a distant prior batch do not document current stock. The date should relate to the batch being purchased.
✗ Not sufficient for research-grade GLP-3 RT
HPLC-only COA with no mass spectrometry HPLC reports the relative purity of the main peak; it does not confirm the identity of that peak without a reference standard or complementary MS data.
Vendor-issued internal COA only Certificates generated solely by the vendor's own laboratory lack independent verification.
Floor purity claims without lot-specific values ("≥95% purity") A marketing floor is not analytical documentation. Lot-specific measured values are what matter for experimental reproducibility.
Verified Purity Data

Evo Peptides GLP-3 RT purity by current variant

The figures below are lot-specific HPLC purity values from independent third-party COAs for material currently offered. Full COAs (including mass spectrometry) are accessible on the product page and at evopeptidesus.com/coas prior to purchase.

GLP-3 RT 15 mg
99.27%
HPLC Purity
3rd Party COA
GLP-3 RT 20 mg
99.85%
HPLC Purity
3rd Party COA
GLP-3 RT 30 mg
99.91%
HPLC Purity
3rd Party COA
Why the difference between ~95% and ≥99.9% matters experimentally

At 95% purity, up to 5% of a lipidated triple-agonist sample may consist of uncharacterized impurities — truncation sequences, de-lipidated species. These can shift dose-response curves and increase inter-experiment variability. At 99.9% the impurity floor is far lower. For comparative designs in which the glucagon-receptor contribution is a primary variable, the difference is experimentally relevant.

Sourcing Due Diligence

Red flags when evaluating GLP-3 RT suppliers

The following patterns suggest a supplier is unlikely to meet the analytical standards required for reproducible research. Any one of them warrants additional scrutiny before purchase.

1
No COA accessible on the product page before purchase A legitimate supplier makes the current lot COA available for review prior to payment. Requiring a post-order request removes the ability to evaluate documentation before committing.
2
Price substantially below the typical research range (~$85–$130 per vial) Synthesis of a 39-residue lipidated peptide plus independent third-party analysis carries real cost. Material priced far below the prevailing range often reflects reduced synthesis or analytical standards.
3
Use of "pharmaceutical grade" or "clinical grade" claims These terms have no regulatory definition for RUO research peptides. Such language is marketing rather than a verified manufacturing standard.
4
Conflation of GLP-3 RT with GLP-2 TRZ The two compounds have different sequences, molecular weights, lipid modifications, and receptor profiles. A supplier that treats them as interchangeable or "similar" is unlikely to characterize either correctly.
5
Payment restricted solely to peer-to-peer apps Inability to process standard credit/debit methods can indicate operational limitations or elevated risk. Research-scale suppliers typically support conventional payment processors.
6
No verifiable domestic fulfillment address Suppliers that exist only as websites without a verifiable US address offer limited accountability. Claims of rapid domestic delivery combined with no domestic footprint often indicate overseas drop-shipping of material of unknown provenance.
7
Implied therapeutic or human-use language on the site Any description of GLP-3 RT in terms of human clinical outcomes or benefits places the vendor outside the RUO framework and signals that the primary customer base is not laboratory researchers.
Comparison

GLP-3 RT vs GLP-2 TRZ: why sourcing standards differ

When both GLP-3 RT and GLP-2 TRZ are sourced for parallel research designs, the same COA expectations apply to both. The underlying identity-verification challenge is greater for the unapproved triple agonist. See the GLP-2 TRZ vs GLP-3 RT research comparison for study-design considerations.

Sourcing comparison: GLP-2 TRZ vs GLP-3 RT
Factor GLP-2 TRZ GLP-3 RT
FDA approval statusApprovedInvestigational
Pharmacopeial reference standardExistsDoes not exist
MS identity confirmationImportantNon-negotiable
Receptor targetsGLP-1 + GIP (dual)GLP-1 + GIP + Glucagon (triple)
Market maturityHigher — more established vendorsLower — newer synthesis target
Typical research price range~$60–$90 / vial~$85–$130 / vial
Minimum HPLC purity (research grade)≥99% for both
Current Evo Peptides lot puritySee product page99.27% / 99.85% / 99.91% by variant
SourceGLP-2 TRZ product pageGLP-3 RT product page
Laboratory Handling

Storage and reconstitution of lyophilized GLP-3 RT

Lipidated peptides require careful handling. The C18 fatty-acid modification that extends half-life in vivo models also increases susceptibility to aggregation under suboptimal reconstitution conditions. The protocols below apply to lyophilized material as supplied. For broader guidance across peptide classes, see the peptide storage and reconstitution guide. Bacteriostatic water is available for laboratory reconstitution kits.

❄ Storage — Lyophilized

  • Store at −20 °C, away from frost-cycle zones
  • Protect from light (amber vial preferred)
  • Keep desiccant in the storage container
  • Avoid long-term storage at 4 °C in lyophilized form
  • Minimize temperature cycling before first reconstitution

⚗ Reconstitution

  • Use bacteriostatic water or 0.1–1% sterile acetic acid
  • Add solvent slowly down the vial wall — do not vortex
  • Swirl gently; allow 5–10 min for full dissolution
  • After reconstitution: 4 °C, use within 14 days
  • Aliquot into single-use volumes before −80 °C storage
Freeze-thaw cycles reduce bioactivity

Each freeze-thaw cycle after reconstitution degrades the compound. Prepare single-use aliquots before freezing. Once thawed, do not refreeze. Snap-freezing in liquid nitrogen is preferred when −80 °C long-term storage is planned.

Research Context

What GLP-3 RT is studied for in preclinical research

Understanding the research questions asked of GLP-3 RT clarifies which analytical requirements are most critical for a given protocol. The compound is examined across several mechanistic axes, each with different endpoint needs. See the GLP-3 RT research guide for mechanism detail and cited Phase 2 data.

Energy expenditure and thermogenesis

The glucagon-receptor component distinguishes GLP-3 RT from dual agonists. In rodent models, glucagon-receptor agonism is associated with increased energy expenditure beyond GLP-1 agonism alone, involving brown-adipose-tissue thermogenesis and hepatic glucose oxidation. Lipidation confirmation in the COA is especially relevant for these endpoints because de-lipidated material exhibits altered pharmacokinetics.

Body composition in diet-induced obesity models

Triple-agonist studies in diet-induced obesity models examine fat-mass reduction relative to lean-mass preservation. Parallel designs comparing GLP-3 RT with GLP-2 TRZ allow attribution of differences to the glucagon-receptor axis. Sourcing both compounds from the same supplier under consistent analytical methods reduces a common source of experimental confound.

Hepatic lipid metabolism models

Glucagon-receptor activation has established roles in hepatic glucose production and emerging roles in hepatic lipid handling. Studies in NASH/NAFLD models frequently use liver triglyceride quantification and histological scoring as primary readouts. Intact lipidation is again relevant because pharmacokinetic differences alter hepatic exposure.

Comparative pharmacology across the incretin class

Head-to-head designs that place GLP-3 RT and GLP-2 TRZ in the same model system allow direct attribution of effects to the glucagon-receptor contribution. For mechanistic comparison and study-design notes, see GLP-2 TRZ vs GLP-3 RT: research comparison.

Where to Source

Where to source research-grade GLP-3 RT in 2026

Evo Peptides offers GLP-3 RT in 15 mg, 20 mg, and 30 mg formats with independent third-party HPLC and mass-spectrometry verification on every batch. Lot-specific COAs are available for review on the product page before purchase. Orders placed before 3:00 PM CST ship the same business day from Wisconsin. Standard credit/debit processing and cryptocurrency are accepted.

GLP-3 RT
Triple GLP-1 / GIP / Glucagon Receptor Agonist · LY3437943 · Research Use Only
≥99% HPLC — lot-specific MS identity confirmed 3rd-party COA included Lyophilized powder Wisconsin fulfillment Same-day before 3 PM CST
$89.95 / vial · free shipping over $200
View GLP-3 RT Product Page View COAs
Same-day shipping · 3:00 PM CST cutoff · Wisconsin · Free shipping over $200

Orders placed before 3:00 PM CST ship the same business day from Wisconsin to all 50 states. Credit card, debit card, and cryptocurrency accepted. Free shipping on orders over $200. All material is supplied under Research Use Only labeling.

FAQ

Frequently asked questions

Is GLP-3 RT legal to buy for research in the USA?

Yes, under Research Use Only conditions. GLP-3 RT (LY3437943) is not a scheduled or controlled substance. US research peptide suppliers may offer it under RUO labeling for in vitro laboratory work. It is not FDA-approved and may not be purchased or used for human or veterinary administration. The FDA compounding pharmacy framework does not govern RUO research vendors and does not determine availability through this channel.

What must a GLP-3 RT COA contain?

At minimum: HPLC purity of 99% or higher with chromatogram available; mass spectrometry identity confirmation (observed mass consistent with the intact sequence including lipid, ~4,900 Da); the independent testing laboratory's name; a batch/lot number matching the vial; and an issue date within approximately 12 months. A purity-only COA is insufficient for GLP-3 RT. See the COA evaluation guide for additional detail.

Why is GLP-3 RT identity verification harder than for approved dual agonists?

Approved dual agonists have FDA-recognized pharmacopeial reference standards. GLP-3 RT (LY3437943) has no FDA approval and therefore no such standard. Mass spectrometry of the sequence and lipid modification is the practical identity confirmation method; HPLC alone cannot establish identity without a reference compound.

What is GLP-3 RT and how does it differ from GLP-2 TRZ?

GLP-3 RT (LY3437943) is a triple agonist of GLP-1, GIP, and glucagon receptors currently in Phase 3 clinical development. GLP-2 TRZ is a dual GLP-1/GIP agonist that is FDA-approved. The compounds differ in sequence, molecular weight, lipid modification, and receptor profile. See the GLP-3 RT research guide for mechanistic context.

How should GLP-3 RT be stored and reconstituted in the laboratory?

Store lyophilized material at −20 °C, protected from light and moisture. Reconstitute with bacteriostatic water or 0.1–1% sterile acetic acid — add solvent slowly, swirl gently, do not vortex. After reconstitution store at 4 °C and use within 14 days, or aliquot and freeze at −80 °C. Avoid freeze-thaw cycles. Full protocols are in the peptide storage guide.

Can GLP-3 RT and GLP-2 TRZ be sourced from the same supplier for parallel studies?

Yes. For designs that compare dual versus triple receptor agonism, sourcing both compounds from the same supplier under consistent COA methodology reduces analytical confounds across study arms.

What is the typical price range for research-grade GLP-3 RT with third-party COA?

Material from US suppliers that provide independent third-party testing typically falls in the approximate $85–$130 per vial range. Pricing substantially below this range often warrants closer examination of the COA and testing methodology.

Reference

Glossary

Triple Incretin Agonist
A peptide engineered to activate GLP-1, GIP, and glucagon receptors simultaneously with a single molecule — distinct from dual agonists (GLP-1 + GIP) or single agonists (GLP-1 only).
Lipidated Peptide
A peptide carrying a covalently attached fatty-acid chain (C18 in GLP-3 RT) that enables albumin binding and extended half-life in research models.
HPLC Purity
High-performance liquid chromatography purity — the percentage of total UV-absorbing material represented by the main peak. Confirms purity but not identity without a reference standard or complementary MS.
Mass Spectrometry
Technique that confirms molecular identity via mass-to-charge measurement. For GLP-3 RT, ESI-MS or MALDI-TOF confirmation of the expected mass is the primary identity test in the absence of a pharmacopeial standard.
COA
Certificate of Analysis — a document from an independent third-party laboratory reporting identity, purity, and quality for a specific production batch. Vendor-internal certificates are not equivalent.
RUO
Research Use Only — the legal designation indicating a product is sold exclusively for in vitro laboratory research and is not for human or veterinary use.
Glucagon Receptor
Class B GPCR expressed primarily in hepatocytes; the third target of GLP-3 RT (absent in GLP-2 TRZ) and the axis associated with additional energy-expenditure effects in preclinical models.
Selected references
  1. Jastreboff AM, et al. Triple-Hormone-Receptor Agonist for Obesity — A Phase 2 Trial. New England Journal of Medicine, 2023. nejm.org/doi/full/10.1056/NEJMoa2301972
  2. Eli Lilly. Phase 3 Study Comparing Triple and Dual Receptor Agonists in Adults With Obesity (TRIUMPH-5), NCT06662383. clinicaltrials.gov/study/NCT06662383
  3. FDA. Bulk Drug Substances Used in Compounding Under Section 503A of the FD&C Act. fda.gov